Effect of Bacillus amyloliquefaciens as probiotic on total colonies of bacteria, pH and cellulase activity in the small intestine of domestic chicken

This study aimed to determine the effect of the addition of Bacillus amyloliquefaciens into drinking water on the total colonies of Bacillus amyloliquefaciens, Lactobacillus sp, pH, and cellulase activity in the small intestine of domestic chicken in starter period. This study was used 200 days old of domestic chicken CP 808 kept for 8 weeks. Type of cage used 20 units cage measuring 85 cm x 70 cm x 30 cm which each unit contained 10 chickens. The study used the Completely Randomized Design (CRD) with 4 treatments in 5 replications. Treatment was the addition of B. amyloliquefaciens into drinking water (without B. amyloliquefaciens, 45 x 109 CFU/ml, 65×1010 CFU/ml, and 43 x 1012 CFU/ml). The variables observed were the total colonies of B. amyloliquefaciens, Lactobacillus sp, pH, and cellulase activity in the small intestine of domestic chicken. The results showed that addition of B. amyloliquefaciens in drinking water of domestic chicken increased the total colonies of B. amyloliquefaciens, Lactobacillus sp, and cellulase activity in the small intestine, but did not affect the pH. The highest total colonies (B. amyloliquefaciens and Lactobacillus sp) and cellulase activity were found on the addition of B. amyloliquefaciens at a level of 43 x 1012 CFU/gram. Meanwhile, the pH intestine added B. amyloliquefaciens tended to be lower than without B. amyloliquefaciens.